Abstract:
Expression and Significance of CD4+CD25+FOXP3+Regulatory T Cells in Patients withAstrocytomaYingzi LIU1, Baoen SHAN2, Yangying SONG2, Qiaoxia LI2, Lei ZHANG1, Xuexin ZHANG1Correspondence to: Baoen SHAN, E-mail: baoenshan@yahoo.com.cn1Department of Neurosurgery, The 4th Hospital Affiliated to Hebei Medical University, Shijiagzhuang 050011, China2Research Center, The 4th Hospital Affiliated to Hebei Medical University, Shijiagzhuang 050011, ChinaAbstract Objective: To investigate the expression of regulatory T lymphocytes in human astrocytoma and its correlation withthe progression of astrocytoma. Methods: Data were collected from astrocytoma patients treated in The 4th Hospital Affiliated to HebeiMedical University between September 2008 and June 2010. The expression of CD4+CD25+FOXP3+regulatory T lymphocytes ( Treg )in the tumor tissue and blood were detected by flow cytometry ( FCM ). The expression of transforming growth factor β1 ( TGFβ1 )was detected by ELISA test. Data were analyzed by SPSS software 13.0. Results: A total of 92 patients ( 54 males and 38 females, witha mean age of 51.4 years ) who underwent surgical treatment were divided into three groups according to the pathological grade ( 42grade Ⅳ cases, 21 grade Ⅲ cases, 29 grade Ⅱ cases. The controled brain tissue was obtained from 8 patients who underwent a tempo-ral lobectomy for seizures. There were significant differences in Treg cells between astrocytoma patients and the controls ( l0.67 % ±0.12 % ) both in the brain tissue ( 24.17 % ± 2.54 % ) and in the blood ( 9.34 % ± 2.13 % ) ( P < 0. 01 ). The expression of Treg in astro-cytoma was positively correlated with the tumor grade ( P < 0. 05 ). The expression of TGF β1 in the blood of glioma patients was posi-tively correlated with Treg cells ( r = 0.564, P = 0. 001 ). Conclusion: Our study showed significant increase of Treg cells in the tumortissue and peripheral blood of patients with astrocytoma. The role of Tregs in the tumor biology of gliomas might have potential thera-peutic implications.Keywords Astrocytoma; CD4+CD25+FOXP3+regulatory T cell; Tumor-infiltrating lymphocyte;Peripheral blood mononuclear cell